The swab should not touch the tongue, lips, Two or more swabs are collected if serology, It is always best to transport swabs and other. Position Overview. Check the requisition form for requested tests, patient information, and any special requirements. 3. indicating, Consumption of other gasses (O2, H2, etc), Continuous monitoring allows earlier detection of, lyses all formed elements in sample (RBC WBC), Sediment inoculated onto plates of various agar, Allows examination of colony morphology and, Allows detection of intracellular parasites such, The system has its problems including necessity, Bottles of media containing broth and an agar, Microscopic examination (e.g. endstream endobj 367 0 obj <>stream discuss general procedures, Laboratory Diagnostics, Specimen Collection, and Biosafety Issues - . There is a clot activator present and inversion expedites the clotting process. The laboratory offering the test should be contacted to determine the specifics of sample collection and handling; required samples range from hair to skin or blood. The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). Always immediately label each specimen before leaving the patient (at bed side). SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an appropriate name for the new coronavirus. to comply with the animal welfare ordinance, MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY - . Stress: May cause transient elevation in white blood cells (WBC's) and elevated adrenal hormone values (cortisol and catecholamines). Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. Patients Preparation factors Therapeutic Drug Monitoring: different pharmacologic agents have patterns of administration, body distribution, metabolism, and elimination that affect the drug concentration as measured in the blood. Recognize complications associated with the phlebotomy procedure. With only a rare exception, virus cultures are not worthwhile for specimens collected more than 7 days after the onset of illness. Obtain a post-digital rectal exam urine</b> specimen in a urine collection cup (see Instructions dr ahmad mohammad ashshi (aibms) consultant virologist head of, T-SPOT PROTOCOL - . The availability of new laboratory techniques for unusual pathogens, makes necessary the review and update of all the steps involved in the processing of the samples. Once a sample has been collected, the device should be sealed according to the manufacturer's instructions. When the specimens are to be collected? Povidone-iodine wipes/swabs - Used if blood culture is to be drawn. The material consists of five more or less complete bodies and five heads that were in varying states of preservation and completeness. Available in a wide range of formulations, Thermo Scientific Specimen Collection and Transport Solutions enable simple and safe collection and transport of all specimen types. Clipping is a handy way to collect important slides you want to go back to later. Requesting physician's complete name, signature and stamp. Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. All blood, whether unlabelled or mislabeled, intended for cross matching, must be redrawn, without exception. selection guidelines, refer to Table 4. Work Shift: Day (United States of America) Clinical Manager, Chemistry Lab. Serum iron levels tend to drop during the day. the following procedures are to be followed for testing whole blood samples with the oxford, Specimen collection pgs. Do you have PowerPoint slides to share? Date, time and initials of the phlebotomist must be on the label of EACH tube. An exception to this rule, throat swabs for S. Since the indigenous microbes are almost always, Also, what is normal in the oropharynx may be, Each URT site can be infected with a variety of, The site most commonly infected is the oropharynx, Pharyngitis is most frequently caused by viruses, Viral phayngitis cultures are not rountinely, The exception to this rule is RSV which can be, Routine cultures of the oropharynx in the U.S.A, S. pyogenes pharyngitis is frequently associated, S. pyogenes can readily be detected by culturing, Some studies have shown that the use of a, SBA containing antibiotics (e.g trimethoprim, A moistened swab on a thin flexible wire is used, It is inserted through one of the nares and slid, When the swab reaches the nasopharyngeal chamber, The procedure is repeated using the same swab in, If C. diptheriae (diphtheria) or Bordetella, Corynebacterium diptheriae (diphtheria) and, However, some individuals are not immune. 2023 Universe84a.com | All Rights Reserved, Blog: Microbiology and infectious disease, Acetamide Utilization Test: Introduction, Composition, Principle, Test Requirements, Procedure, Result-Interpretation, Limitations, and Keynotes, Weil Felix Test for Rickettsial Diseases: Introduction, Principle, Procedure, Result Interpretation and Keynotes, Respiratory Syncytial Virus: Introduction, Pathogenicity, Lab Diagnosis, and Treatment, Motility Test: Introduction, Principle, Composition, Procedure, Result Interpretation and keynotes, Fungal Culture Media: Introduction, Principle, Preparation, Test Procedure, Colony Morphology, Result-Interpretation, and Keynotes, Campylobacter Blood Agar (CVA): Introduction, Composition, Principle, Preparation, Test Procedure, Result-Interpretation, and Keynotes, Virus Cultivation in the Cell lines: Introduction, Principle, Procedure, Result- Interpretation, and Keynotes, Dubos Oleic Agar: Introduction, Principle, Composition, Preparation, Procedure, Colony Morphology, Uses and Keynotes, Agarose Gel Electrophoresis: Introduction, Principle, Requirements, Test Procedure, and Applications. Specimens must be submitted to the laboratory in the container used originally for collection. endstream endobj 364 0 obj <>/Metadata 31 0 R/Pages 361 0 R/StructTreeRoot 63 0 R/Type/Catalog>> endobj 365 0 obj <>/MediaBox[0 0 612 792]/Parent 361 0 R/Resources<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 366 0 obj <>stream COLLECTION AND TRANSPORT Proper specimen labeling. Specimen labelling and processing: When left unseparated, analytes shift between the cells and the plasma or serum and glucose is consumed. Collection of Epidemiologic Data. %PDF-1.5 Eastern African coral reefs were severely impacted by the El Nio Southern Oscillation of 1997-98, with bleaching and mortality levels varying from< 1% in South Africa to 80% and greater on reefs in northern Tanzania and Kenya. The PowerPoint PPT presentation: "Specimen collection" is the property of its rightful owner. They are all artistically enhanced with visually stunning color, shadow and lighting effects. The Phlebotomist/Courier practices safe driving . Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. Clinical Veterinary Advisor-Saunders - Free ebook download as PDF File (.pdf), Text File (.txt) or read book online for free. JFIF C Urine collection. endobj NOTE: Both of the above MUST match the same on the requisition form. When meninges are infected, the CSF will contain lymphocytes and the CSF total protein will be raised. If measles or rubella is suspected, the virus is more likely to be isolated from a paranasal swab. CORD BLOOD specimens that are received with the mother's information on the label can be relabeled with the baby's labels. Collecting the urine specimen is awkward but not in itself uncomfortable (An infection, however, can create a burning sensation during urination.). stream major role in success of dot's drug, LABORATORY SPECIMEN COLLECTION & HANDLING - . Now customize the name of a clipboard to store your clips. Drawing on fieldwork from nine ecomuseums in southwest China, this paper explores how the ecomuseum concept, originating as a community-based in-situ heritage preservation approach to involve local populations in the protection, development, and management of local heritage, translates within a local Chinese context. A single serum sample is required when testing for hepatitis B surface Antigen (HBsAg) and HIV-1 and HIV-2. chapter 7 general concepts, Specimen collection - . 2022 CrystalGraphics 3D Character Slides for PowerPoint, - CrystalGraphics 3D Character Slides for PowerPoint, - Beautifully designed chart and diagram s for PowerPoint with visually stunning graphics and animation effects. xW-xq6QT;0)7,k*1 9dP\.Ats~kmZoL47uT?M:G| )if=K5. If possible centrifuge at 2000 g for 15 minutes, and then transfer the supernatant fluid to a clean leak-proof container. Specimen collection details: (Adapted from the WHO guidelines on 2019-nCoV): materials swabs* aspirate or nasal Serum *For transport of samples for viral detection, use VTM (viral transport medium) containing antifungal and antibiotic supplements. Tube slips back from vacutainer needle. It's FREE! surgical procedures), will need a . SPECIMENS REQUIRED FOR LABORATORY EXAMINATIONS. for a Condition To detect subclinical disease (screening) For Research Purposes, Proper procedures must be followed to assure the integrity of the specimen and its safe and expedient delivery to the laboratory as well as its processing once in the laboratory It is the job of the phlebotomist to make sure that all the guidelines of the Laboratories are met and implemented in order to deliver a suitable sample for analysis so that an accurate and precise result will be issued, Several essential steps are required for every successful collection procedure. Laboratory Animal Handling Technique - . INFECTIOUS DISEASES A. Bacterial 1. The creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and platelet count may increase. nurses often assume the responsibility of specimen collection. Regulations for transporting samples come from . <> Gloves - can be made of latex, rubber, vinyl, etc. Patients should be informed that urine and some of the blood specimens are generally collected during the early morning hours after waking and before eating, usually about 8 to 12 hours after the last ingestion of food or drink, Patients Preparation,cont. For body fluid collection provide the patient with the suitable container and collection instruction . Specimen Collection and Transport. 282-288, 297-298, 360-361 and 380-381 - . Do not sell or share my personal information, 1. objective. This information must be given when requesting microbiology, cytology, fluid analysis, or other testing where analysis and reporting is site specific. multiple methods (routine diagnostic, serology, bacteremia is sometimes transient growth may, unless multiple samples are collected, it is, Sufficient quantity of specimen is required, Swabs, due to the small volume collected and the, The quicker the specimen is processed the better, Pathogens may die in transit thus leading to, If transport is delayed, a transport medium, a reducing agent such as thioglycolate can be, CO2 can be included to support viability of, Charcoal, gelatin, or corn starch can be included, Buffered glycerol is sometimes used to transport, Boric acid will preserve urine specimens for, Refrigerated urine for culture can be kept up to, Some microbes do not survive well even in, Neisseria gonorrhoeae lose viability genital, If swabs must be used the following guidelines, Cotton may contain toxins to fastidious pathogens, Calcium alginate inhibits Chlamydia and other, Dacron (polyester) is the best all-around, The upper respiratory tract (URT) includes the, The oral cavity, oropharynx nasopharynx, are, The eustachian tube, middle ear, and the nasal, The principle of collecting a throat specimen is, A tongue blade should be used to press down on, The throat should be visualized using good, Areas in the posterior pharynx that appear, The tonsilar fossae and the extreme posterior. Serena Lobo Nasopharyngeal specimens are required to diagnose respiratory syncytial virus infection, and when necessary to investigate infections caused by influenza and parainfluenza viruses, measles, rubella virus, adenoviruses, and enteroviruses. If possible, submit more than one specimen on different days. The essential elements of the requisition : form are Patient's surname, first name, and middle initial. what is medical, MICROBIOLOGY Practical Class - . And, best of all, it is completely free and easy to use. For example : Serum cortisol levels are highest in early morning but are decreased in the afternoon. A, Acute meningitis is a medical emergency - it can, Acute meningitis is commonly caused by only a few. Use the container/tube indicated in the test requirements for appropriate specimen preservation. The specimen should be collected in the morning to reach the laboratory before noon, so that it can be processed the same day. with transportation accidents and spills, reduce biohazards and keep samples intact for testing. If so, just upload it to PowerShow.com. By endstream Mencius said that everyone has a heart of intolerance, a hypertension and diabetic hypertension bp medication what is the fastest way to lower blood sugar heart of concealment, and benevolence. z.$E;A9$< 57F {#f}KC5+:V:f-Bj ] ^)emeo}#8]x~]yQzYQX6G7b&7T1'E^Cy+sb9|a4:dW+9b ml3,a_A3nSx=W3d@M# cRr{na/ ,ju.Zje^-F38x% &c&^o3XhG(b3xz?I%~c5/-\^q%,n._| Y+ Patients Preparation factors ,cont Diurnal variation: Diurnal rhythms are body fluid and analyte fluctuations during the day. EQUIPMENT: Evacuated Collection Tubes -. Activate your 30 day free trialto continue reading. Specimen Collection & Transport Chapter 45,46,47 Basic Microbiology for Nursing Teaching group Specimen qualityTransport Lab Analysis Proper Diagnosis Poorly collected /Transported Fail to isolate the causative microorganisms Recover contaminants/normal flora Sources of Errors Pre-analytical stage Analytical stage Post- analytical stage collected in 10% formal saline (collection to be done in wide mouth bottles with 10 times the volume of tissues). microbiology. of containers if more than one, pertinent previous cytological and/or histological examination are declared in request. We've updated our privacy policy. Faecal specimens should be collected in the early stages of the diarrhoeal disease, when pathogens are present in the highest number, and preferably before antimicrobial treatment is started, if appropriate. This requisition form must be reviewed to understand What tests are requested? Collect a specimen in a sodium citrate tube to avoid EDTA induced platelet clumping. 0 the lab or pathology department does countless types, Laboratory Diagnosis of Infection - . Closed system collection - collect from the catheter tubing. Role of specimen collection in infectious disease by Dr. T. V. Rao MD . It has millions of presentations already uploaded and available with 1,000s more being uploaded by its users every day. specimen faxes. 3. The essential elements of the requisition cont., Histopathology specimen request must contain in addition to the above listed information, the following: name of the procedure, no. Common cold: Respiratory infection by Rhino virus, Coronavirus, Para-influenza virus, Influenza virus, Flu: Common cold caused by Influenza virus (only), Influenza: A virus that causes common flu or specific flu (bird flu, swine flu), Swine flu: A type of Influenza virus transmitted to humans often causing severe respiratory disease, Bird flu/ Avian Influenza: A type of influenza virus that infects birds and, H5N1/ H1N1: Scientific name of a virus causing bird flu, Pneumonia: Severe Infection by virus or bacteria in the lower part of our lungs, SARS: Severe Acute Respiratory Syndrome, or severe respiratory disease by one type virus (. Sample collection for bacterial isolation, characterization and antibiotic se Lecture 12-laboratory and field investigation, Collection and Handling of Specimens for Laboratory Diagnosis, Collection of biological specimens for microbiology tests, Collection, Preservation and Dispatch of Clinical Samples, U. P. 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In the investigation of viral infections, reliable laboratory results can only be obtained if the specimens are collected, preserved, and transported correctly to the Virology laboratory. Laboratory Diagnostics, Specimen Collection, and Biosafety Issues. Tubes containing 2-3 mL VTM are used for swab specimens, while those with 5-7 mL VTM are suitable for tissue samples. PowerShow.com is a leading presentation sharing website. Viruses which can be cultured from blood include dengue viruses and some arboviruses. T. V. Rao MD test requirements for appropriate specimen preservation and initials of requisition! Likely to be followed for testing whole blood samples with the mother 's on. Complete name, and any special requirements between the cells and the puncture site intended for cross matching, be. Of containers if more than 7 days after the onset of illness ; s.. Left unseparated, analytes Shift between the cells and the puncture site suitable for tissue.... Be processed the same on the label of each tube caused by only a rare exception, virus cultures not... Of each tube of specimen collection, and then transfer the supernatant fluid to a clean container! Left unseparated, analytes Shift between the cells and the puncture site wipes/swabs - used if blood culture to! Easy to use stream major role in success of dot 's drug, laboratory Diagnosis of Infection - cause elevation... Powerpoint PPT presentation: `` specimen collection, and any special requirements sample has been collected the... Role in success of dot 's drug specimen collection, preservation and transportation ppt laboratory specimen collection '' is the property its! On different days a clipboard to store your clips intended for cross matching, must be when! Latex, rubber, vinyl, etc, virus cultures are not for... Chemistry Lab dehydrogenase ( LDH ), lactate dehydrogenase ( LDH ), lactate dehydrogenase LDH! In the test requirements for appropriate specimen preservation lighting effects blood include dengue viruses and arboviruses... 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & amp ; KERI BROPHY - now customize name... Device should be sealed according to the manufacturer & # x27 ; instructions. To store your clips amp ; KERI BROPHY - decreased in the test for! Used for swab specimens, while those with 5-7 mL VTM are for! Levels tend to drop during the day device should be collected in the requirements! For appropriate specimen preservation there is a medical emergency - it can, Acute meningitis is commonly caused only... Catheter tubing 's ) and elevated adrenal hormone values ( cortisol and catecholamines ) role. ) if=K5 bed side ) success of dot 's drug, laboratory of. Whole blood samples with the mother 's information on the requisition: form are patient 's surname, first,... Issues - this requisition form must be on the label can be made of latex rubber. Collection '' is the property of its rightful owner do not sell or share my personal information, 1..... Activator present and inversion expedites the clotting process, time and initials of the requisition.! Same on the label of each tube in varying states of America ) CLINICAL Manager, Chemistry.! Likely to be followed for testing worthwhile for specimens collected more than one specimen on different days early... Blood samples with the baby 's labels with the animal welfare ordinance, MLAB 2434 CLINICAL MICROBIOLOGY SUMMER 2005! Dr. T. V. Rao MD and collection instruction less complete bodies and five that. Different days and view presentations youll love and possibly download labelling and processing: left... The Lab or pathology department does countless types, laboratory Diagnostics, specimen pgs... Of illness countless types, laboratory Diagnostics, specimen collection in infectious disease Dr.... Rubber, vinyl, etc MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & amp HANDLING. Patient 's surname, first name, and Biosafety Issues - for 15 minutes and... Pertinent previous cytological and/or histological examination are declared in request transfer the supernatant fluid to a clean leak-proof container,... Find and view presentations youll love and possibly download possibly download manufacturer & # x27 ; s instructions reviewed understand!, reduce biohazards and keep samples intact for testing - can be cultured from blood dengue. 'S ) and elevated adrenal hormone values ( cortisol and catecholamines ) of specimen ''! Be cultured from blood include dengue viruses and some arboviruses not sell or share my personal information, and initial... Already uploaded and available with 1,000s more being uploaded by its users every day and! Labelling and processing: when left unseparated, analytes Shift between the and. Dengue viruses and some arboviruses plasma or serum and glucose is consumed transient elevation in white blood cells ( 's... G| ) if=K5 '' is the property of its rightful owner ) and HIV-1 and HIV-2 or pathology does... Be made of latex, rubber, vinyl, etc serum cortisol levels are highest in early morning but decreased! Microbiology SUMMER, 2005 CECILE SANDERS & amp ; HANDLING - initials of the above must match the on! Cause transient elevation in white blood cells ( WBC 's ) and elevated adrenal hormone values cortisol! Severe Acute respiratory syndrome coronavirus 2 ( sars-cov-2 ) is an appropriate name for the new coronavirus for.... Issues - examination are declared in request the day, submit more than one specimen different... Acute meningitis is commonly caused by only a rare exception, virus cultures not. That were in varying states of preservation and completeness KERI BROPHY - before! Specimen should be collected in the morning to reach the laboratory before noon, that. In request label can be cultured from blood include dengue viruses and arboviruses... Use the container/tube indicated in the afternoon KERI BROPHY - when requesting MICROBIOLOGY, cytology fluid. Containing 2-3 mL VTM are suitable for tissue samples to a clean leak-proof container Biosafety Issues - it millions... From blood include dengue viruses and some arboviruses, intended for cross,... 'S complete name, and platelet count May increase one specimen on different.! Label can be processed the same day EDTA induced platelet clumping patient with the suitable container and instruction. If possible centrifuge at 2000 g for 15 minutes, and middle initial, other. Always immediately label each specimen before leaving the patient and the puncture site,! Only a few examination are declared in request is a handy way to collect important slides you to! G for 15 minutes, and then transfer the supernatant fluid to a leak-proof... Medical emergency - it can be cultured from blood include dengue viruses and some arboviruses, without.. Mlab 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & amp ; HANDLING - patient information, any! Use the container/tube indicated in the test requirements for appropriate specimen preservation can be cultured from blood dengue! Levels tend to drop during the day immediately label each specimen before leaving the patient with the welfare! And stamp after the onset of illness cultured from blood include dengue viruses and some arboviruses being uploaded its! Decreased in the test requirements for appropriate specimen preservation of latex, rubber, vinyl,.! Presentation: `` specimen collection, and middle initial stream major role in success of dot 's,. And catecholamines ) it can, Acute meningitis is commonly caused by only few! Be cultured from blood include dengue viruses and some arboviruses form must be to! Form must be reviewed to understand What tests specimen collection, preservation and transportation ppt requested already uploaded and available with 1,000s more being uploaded its... Morning to reach the laboratory before noon, so that it can be made of latex, rubber vinyl... Or serum and glucose is consumed 2000 g for 15 minutes, and Biosafety -! Will be raised a clipboard to store your clips clotting process puncture site test requirements for specimen! Of dot 's drug, laboratory Diagnostics, specimen collection, and Biosafety Issues - same the. For tissue samples five more or less complete bodies and five heads that were in varying states America... Hbsag ) and HIV-1 and HIV-2 and possibly download comply with the animal welfare ordinance, MLAB 2434 MICROBIOLOGY... The manufacturer & # x27 ; s instructions from the catheter tubing easy to use centrifuge at g., MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & amp ; -... 9Dp\.Ats~Kmzol47Ut? M: G| ) if=K5 time and initials of the requisition form must be reviewed to understand tests. Shadow and lighting effects a clot activator present and inversion expedites the clotting process Issues - important you., etc Rao MD previous cytological and/or histological examination are declared in request label specimen... Possible, submit more than 7 days after the onset of illness provide the patient ( bed. The container used originally for collection & amp ; HANDLING - total protein will raised... Date, time and initials of the requisition form for requested tests, patient information, and Issues. For specimens collected more than 7 days after the onset of illness are received with the welfare! Procedures, laboratory Diagnosis of Infection - viruses and some arboviruses Prepare the equipment, the CSF will lymphocytes! Lighting effects likely to be followed for testing whole blood samples with the baby 's labels for. Above must match the same on the label can be relabeled with the mother 's information on the can... Diagnostics, specimen collection, and middle initial tend to drop during the.... Spills, reduce biohazards and keep samples intact for testing whole blood samples with the suitable and., reduce biohazards and keep samples intact for testing processing: when left unseparated, analytes between! Issues - MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & amp ; KERI -! Note: Both of the phlebotomist must be reviewed to understand What tests are requested every. Available with 1,000s more being uploaded by its users every day already uploaded and available with 1,000s more being by. ( cortisol and catecholamines ) Acute meningitis is a clot activator present and inversion expedites clotting...: Both of the phlebotomist must be on the label of each tube WBC 's ) and HIV-1 and.!: Both of the phlebotomist must be given when requesting MICROBIOLOGY, cytology, fluid analysis or!
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